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:: Volume 34, Issue 4 (winter 2024) ::
MEDICAL SCIENCES 2024, 34(4): 401-410 Back to browse issues page
Investigation of carbohydrates, and in-vitro antimicrobial and prebiotic effects of alfalfa extract in comparison with inulin
Morvarid Moradi Chamachar1 , , Nasrin Samadi 2, Mohammadreza Fazeli3 , Mona Salimi4
1- PhD Student, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Unit, Tehran Iran
2- Associate Professor, Drug and Food Control Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran , samadin@tums.ac.ir
3- Professor, Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4- Professor, Department of Physiology and Pharmacology, Pasteur Institute, Tehran, Iran
Abstract:   (333 Views)
Background: The alfalfa plant with the scientific name Medicago Sativa is called the queen of forages due to its high nutritional value. On the other hand, studies have shown that alfalfa has an effect on the intestinal microbial composition and its function. Inulin is also a compound with natural prebiotic properties that is used in various products. The aim of this study was to investigate carbohydrates, and in-vitro antimicrobial and the prebiotic activities of alfalfa extract compared to inulin.
Materials and methods: Extraction from the aerial part of alfalfa was done by maceration method. Then the carbohydrates and prebiotic effect of alfalfa extract compared to inulin on the growth of two bacteria lacticasei bacillus casei and lactiplanti bacillus plantarum and also its effect on the antimicrobial activity of their supernatant were investigated.
Results: Alfalfa extract showed more prebiotic properties than inulin, so that growth and antimicrobial effect of two bacteria, Lacticasei bacillus casei and Lactiplanti bacills plantarum, in the medium containing 2.5% extract were more than the medium containing inulin with the same amount.
Conclusion: In this study, an acceptable prebiotic property was obtained for alfalfa extract compared to the inulin, which makes alfalfa a suitable candidate for further studies to modulate the intestinal microbiome of humans and animals.
 
Keywords: Alfalfa, Probiotic, Prebiotic, Inulin.
Full-Text [PDF 1071 kb]   (135 Downloads)    
Semi-pilot: Experimental | Subject: Microbiology
Received: 2023/12/3 | Accepted: 2024/03/12 | Published: 2024/11/30
References
1. Lorenzo-Leal AC, Palou E, López-Malo A. Evaluation of the efficiency of allspice, thyme and rosemary essential oils on two foodborne pathogens in in-vitro and on alfalfa seeds, and their effect on sensory characteristics of the sprouts. Int J Food Microbiol 2019; 295:19-24. [DOI:10.1016/j.ijfoodmicro.2019.02.008]
2. Kumar A, Rahal A, Chakraborty S, Tiwari R, Latheef SK, Dhama K. Ocimum sanctum (Tulsi): a miracle herb and boon to medical science- A Review. Int J Agron Plant Prod 2013; 4 :1580-9.
3. Samac DA, Austin-Phillips S. Alfalfa (Medicago sativa L.). Agrobacterium protocols 2006:301-12.
4. Watson BS, Lei Z, Dixon RA, Sumner LW. Proteomics of Medicago sativa cell walls. Phytochemistry 2004; 65:1709-20. [DOI:10.1016/j.phytochem.2004.04.026]
5. Liu X, Sun Y, Bian J, Lv M, Han T, Sun H, et al. Phytochemical and chemotaxonomic study on Medicago sativa L.(leguminosae). Biochem Syst Ecol 2018; 80: 55-8. [DOI:10.1016/j.bse.2018.06.008]
6. Nakhei rad MH, Khiyabaniyan asl A, Azari Takami Gh, Razavilar V, Tehrani Sharif M. Effect of probiotic Pediococcus acidilactic and manioligosaccharide and beta-glucan prebiotic on immune salmon and survival parameters against Streptococcus inaei. Vet Res Biol Prod 2020; 132: 40-47. [In Persian]
7. Wang J, Qin C, He T, Qiu K, Sun W, Zhang X, et al. Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs. J Anim Sci Biotechnol 2018; 9: 1-9. [DOI:10.1186/s40104-017-0216-y]
8. Teferra TF. Possible actions of inulin as prebiotic polysaccharide: A review. Food Front 2021; 2:407-16. [DOI:10.1002/fft2.92]
9. Snow Setzer M, Sharifi-Rad J, Setzer WN. The search for herbal antibiotics: An in-silico investigation of antibacterial phytochemicals. Antibiotics 2016; 5:30. [DOI:10.3390/antibiotics5030030]
10. Khan S, Jan G, Bibi H, Sher J, Ullah S, Abidullah S. Phytochemical screening and antimicrobial activity of the Cichorium intybus (Familyasteraceae) and Medicago sativa (Familyfabaceae) Peshawar. Pak J Pharmacognosy Phytochem 2018; 7:603-16.
11. Antonio C, Dolores M, Martínez Carrera DC, Rivera Tapia JA, Portillo Reyes R, Morales Almora P, et al. Detection of polysaccharides in Ganoderma lucidum extracts. Nova scientia2018; 10:247-57. [DOI:10.21640/ns.v10i21.1538]
12. Kato Y, Matsuda T, Hashimoto T. New gel permeation column for the separation of watersoluble polymers. J Chromatogr A 1985; 332: 39-46. [DOI:10.1016/S0021-9673(01)83285-4]
13. Rovkina K, Krivoshchekov S, Guryev A, Yusubov M, Belousov M. Water-soluble polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of flora of Krasnoyarsk krai. Russ J Org Chem 2018; 44: 854-9. [DOI:10.1134/S1068162018070105]
14. Slobodianiuk L, Budniak L, Marchyshyn S, Kostyshyn L, Zakharchuk O. Analysis of carbohydrates in Saponaria officinalis L. using GC/MS method. Pharmacia 2021; 68:339-45. [DOI:10.3897/pharmacia.68.e62691]
15. Starke I, Holzberger A, Kamm B, Kleinpeter E. Qualitative and quantitative analysis of carbohydrates in green juices (wild mix grass and alfalfa) from a green biorefinery by gas chromatography/mass spectrometry. Fresenius J Anal Chem 2000; 367: 65-72. [DOI:10.1007/s002160051600]
16. Chamachar MM, Fazeli MR, Salimi M, Samadi N. Growth promoting activity, anti-biofilm effect, and down regulation of papC and rcsA genes expression by Medicago sativa (alfalfa) extract. Food Bioscience 2022; 50: 102182 [DOI:10.1016/j.fbio.2022.102182]
17. Abobaker A, Alzwi A, Alraied AHA. Overview of the possible role of vitamin C in management of COVID-19. Pharmacol Rep 2020; 72:1517-1528. [DOI:10.1007/s43440-020-00176-1]
18. Darabipour F, Isazadeh Kh, Faazi Ghasemi M, Sadeghi Khamene Tabrizi S. Evaluation of antagonism effects Lactobacillus Acidophilus Lactobacillus palantaromber Z Enterobacteriaceae isolated from plants. Microbe World Magazine 2015 14-39.
19. Gillon AD, Saska I, Jennings CV, Guarino RF, Craik DJ, Anderson MA. Biosynthesis of circular proteins in plants. Plant J 2008; 53:505-15. [DOI:10.1111/j.1365-313X.2007.03357.x]
20. Li Y, Fu X, Ma X, Geng S, Jiang X, Huang Q, et al. Intestinal microbiome-metabolome responses to essential oils in piglets Front Microbiol 2018;9:1988. [DOI:10.3389/fmicb.2018.01988]
21. Liu C, Xue WJ, Ding H, An C, Ma SJ, Liu Y. Probiotic Potential of Lactobacillus Strains Isolated from Fermented Vegetables in Shaanxi, China. Front Microbiol 2022; 12:774903. [DOI:10.3389/fmicb.2021.774903]
22. Lorenzo-Leal AC, Palou E, López-Malo A. Evaluation of the efficiency of allspice, thyme and rosemary essential oils on two foodborne pathogens in in-vitro and on alfalfa seeds, and their effect on sensory characteristics of the sprouts. Int J Food Microbiol 2019; 295:19-24. [DOI:10.1016/j.ijfoodmicro.2019.02.008]
23. Kumar A, Rahal A, Chakraborty S, Tiwari R, Latheef SK, Dhama K. Ocimum sanctum (Tulsi): a miracle herb and boon to medical science- A Review. Int J Agron Plant Prod 2013; 4 :1580-9.
24. Samac DA, Austin-Phillips S. Alfalfa (Medicago sativa L.). Agrobacterium protocols 2006:301-12.
25. Watson BS, Lei Z, Dixon RA, Sumner LW. Proteomics of Medicago sativa cell walls. Phytochemistry 2004; 65:1709-20. [DOI:10.1016/j.phytochem.2004.04.026]
26. Liu X, Sun Y, Bian J, Lv M, Han T, Sun H, et al. Phytochemical and chemotaxonomic study on Medicago sativa L.(leguminosae). Biochem Syst Ecol 2018; 80: 55-8. [DOI:10.1016/j.bse.2018.06.008]
27. Nakhei rad MH, Khiyabaniyan asl A, Azari Takami Gh, Razavilar V, Tehrani Sharif M. Effect of probiotic Pediococcus acidilactic and manioligosaccharide and beta-glucan prebiotic on immune salmon and survival parameters against Streptococcus inaei. Vet Res Biol Prod 2020; 132: 40-47. [In Persian]
28. Wang J, Qin C, He T, Qiu K, Sun W, Zhang X, et al. Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs. J Anim Sci Biotechnol 2018; 9: 1-9. [DOI:10.1186/s40104-017-0216-y]
29. Teferra TF. Possible actions of inulin as prebiotic polysaccharide: A review. Food Front 2021; 2:407-16. [DOI:10.1002/fft2.92]
30. Snow Setzer M, Sharifi-Rad J, Setzer WN. The search for herbal antibiotics: An in-silico investigation of antibacterial phytochemicals. Antibiotics 2016; 5:30. [DOI:10.3390/antibiotics5030030]
31. Khan S, Jan G, Bibi H, Sher J, Ullah S, Abidullah S. Phytochemical screening and antimicrobial activity of the Cichorium intybus (Familyasteraceae) and Medicago sativa (Familyfabaceae) Peshawar. Pak J Pharmacognosy Phytochem 2018; 7:603-16.
32. Antonio C, Dolores M, Martínez Carrera DC, Rivera Tapia JA, Portillo Reyes R, Morales Almora P, et al. Detection of polysaccharides in Ganoderma lucidum extracts. Nova scientia2018; 10:247-57. [DOI:10.21640/ns.v10i21.1538]
33. Kato Y, Matsuda T, Hashimoto T. New gel permeation column for the separation of watersoluble polymers. J Chromatogr A 1985; 332: 39-46. [DOI:10.1016/S0021-9673(01)83285-4]
34. Rovkina K, Krivoshchekov S, Guryev A, Yusubov M, Belousov M. Water-soluble polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of flora of Krasnoyarsk krai. Russ J Org Chem 2018; 44: 854-9. [DOI:10.1134/S1068162018070105]
35. Slobodianiuk L, Budniak L, Marchyshyn S, Kostyshyn L, Zakharchuk O. Analysis of carbohydrates in Saponaria officinalis L. using GC/MS method. Pharmacia 2021; 68:339-45. [DOI:10.3897/pharmacia.68.e62691]
36. Starke I, Holzberger A, Kamm B, Kleinpeter E. Qualitative and quantitative analysis of carbohydrates in green juices (wild mix grass and alfalfa) from a green biorefinery by gas chromatography/mass spectrometry. Fresenius J Anal Chem 2000; 367: 65-72. [DOI:10.1007/s002160051600]
37. Chamachar MM, Fazeli MR, Salimi M, Samadi N. Growth promoting activity, anti-biofilm effect, and down regulation of papC and rcsA genes expression by Medicago sativa (alfalfa) extract. Food Bioscience 2022; 50: 102182 [DOI:10.1016/j.fbio.2022.102182]
38. Abobaker A, Alzwi A, Alraied AHA. Overview of the possible role of vitamin C in management of COVID-19. Pharmacol Rep 2020; 72:1517-1528. [DOI:10.1007/s43440-020-00176-1]
39. Darabipour F, Isazadeh Kh, Faazi Ghasemi M, Sadeghi Khamene Tabrizi S. Evaluation of antagonism effects Lactobacillus Acidophilus Lactobacillus palantaromber Z Enterobacteriaceae isolated from plants. Microbe World Magazine 2015 14-39.
40. Gillon AD, Saska I, Jennings CV, Guarino RF, Craik DJ, Anderson MA. Biosynthesis of circular proteins in plants. Plant J 2008; 53:505-15. [DOI:10.1111/j.1365-313X.2007.03357.x]
41. Li Y, Fu X, Ma X, Geng S, Jiang X, Huang Q, et al. Intestinal microbiome-metabolome responses to essential oils in piglets Front Microbiol 2018;9:1988. [DOI:10.3389/fmicb.2018.01988]
42. Liu C, Xue WJ, Ding H, An C, Ma SJ, Liu Y. Probiotic Potential of Lactobacillus Strains Isolated from Fermented Vegetables in Shaanxi, China. Front Microbiol 2022; 12:774903. [DOI:10.3389/fmicb.2021.774903]
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Moradi Chamachar M, Samadi , N, Fazeli M, Salimi M. Investigation of carbohydrates, and in-vitro antimicrobial and prebiotic effects of alfalfa extract in comparison with inulin. MEDICAL SCIENCES 2024; 34 (4) :401-410
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Volume 34, Issue 4 (winter 2024) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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