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Growth and differentiation of mesenchymal cell-derived cardiomyocytes on biologically active nanofibers: an experimental study
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Zakiye Mokhames1    , Mahmood Dehghani Ashkezari2 , Ehsan Seyedjafari3 , Seyed Morteza Seifati1 |
1- Biology Department, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran
2- Biology Department, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran , mdashkezary@yahoo.com
3- Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran |
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Abstract: (380 Views) |
Background: Dysfunction of the heart cells is one of the main causes of heart failure or lack of properly heart function. In the present study, human placenta extract (PE) along with poly lactic co-glycolic acid (PLGA) and poly hydroxyl butyrate (PHB) were used to make bioactive scaffolds using electrospinning method.
Materials and methods: Electrospinning method was used to make PLGA-PHB and PLGA-PHB-PE nanofibers. Morphological evaluation of PLGA-PHB and PLGA-PHB-PE nanofibers was done using electron microscope. Degradability rate, water absorption capacity, protein and adhesion strength of the scaffolds were investigated. Mesenchymal cells were extracted from adipose tissue. In order to confirm stem cells, CD34, CD45, CD90 and CD105 markers were performed by flow cytometry. Also, the expression of MyoD and Troponin T genes was performed by RT-PCR.
Results: The degradability of PLGA-PHB-PE scaffold was higher compared to PLGA-PHB in different days. Also, water absorption, protein and adhesive strength were higher in PLGA-PHB-PE scaffold compared to PLGA-PHB (p<0.05). Gene expression of MyoD and Troponin T genes was higher in cells grown on PLGA-PHB-PE scaffold compared to PLGA-PHB (p<0.05).
Conclusion: According to the results, it can be concluded that the PLGA-PHB-PE scaffold has a high potential to promote the cardiac differentiation of MSCs and can be used in cardiac tissue engineering for heart muscle repair.
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| Keywords: Nanofibers, Stem cells, Tissue engineering, Mesenchymal cells, Poly lactic-co-glycolic acid, Poly hydroxyl butyrate |
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Full-Text [PDF 1746 kb]
(112 Downloads)
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Semi-pilot: Experimental |
Subject:
Cardiology
Received: 2024/07/28 | Accepted: 2024/11/16 | Published: 2025/09/1
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Mokhames Z, Dehghani Ashkezari M, Seyedjafari E, Seifati S M. Growth and differentiation of mesenchymal cell-derived cardiomyocytes on biologically active nanofibers: an experimental study. MEDICAL SCIENCES 2025; 35 (3) :294-305 URL: http://tmuj.iautmu.ac.ir/article-1-2283-en.html
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