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Research of the effect of cold atmospheric plasma using helium gas on cervical cell lines
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Mahsa Vatani1   , Simzar Hosseinzadeh2  , Amirhossein Sari1 , Azam Rahimpour3 , Hamidreza Ghomi Marzdashti4 |
1- Department of Physics, SR.C., Islamic Azad University, Tehran, Iran
2- Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran - School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , symzar.hosseinzadeh@gmail.com
3- Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran - School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran |
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Abstract: (343 Views) |
Background: Invasive cervical cancer is well-known as the second most common malignancy in women after breast cancer, with an increasing incidence. Here, for the first time, the influence of the corresponding plasma type was examined on the human epithelial carcinoma (HeLa) cell line in the presence of cold plasma of He.
Materials and methods: OES confirmed the development of radical species of N2I2+, N2II1-, He· and O-·. biological impacts were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and Real-Time PCR.
Results: Electron density and temperature were measured using the Stark broadening method at atmospheric pressure in two emission lines. In general, this method is defined based on Stark broadening, which is dependent on temperature and electron density.BY using MTT method, apoptotic effect of the plasma showed the group with the smallest distance from the jet (3 cm) and longest treatment time (3 min) led the cell shape into a collapsed round type, and the cells lost their attachment over time. When measuring the remaining radical developed by DPPH, more radical formation occurred when the incubation time was increased from 30s to 60s. Real-time PCR confirmed the highest levels of p53, Bax and caspase 3/8 expressions after 60 s. The expression of Bcl2 as an anti-apoptotic gene showed a significantly lower value.
Conclusion: Taken together, the results confirmed cold plasma parameters are essential factors that must be optimized to reach therapeutic outcomes in clinical trials.
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| Keywords: Cervical cancer, Cold atomospheric plasma, Radical species, Stark broadening |
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Full-Text [PDF 1674 kb]
(83 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Oncology
Received: 2025/08/17 | Accepted: 2025/11/18 | Published: 2026/07/1
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Vatani M, Hosseinzadeh S, Sari A, Rahimpour A, Ghomi Marzdashti H. Research of the effect of cold atmospheric plasma using helium gas on cervical cell lines. MEDICAL SCIENCES 2026; 36 (2) :154-165 URL: http://tmuj.iautmu.ac.ir/article-1-2441-en.html
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