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The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus
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Amin Dinarvand1   , Mehrdad Hashemi  2, Rasool Dinarvand3 , Shabnam Movaseghi4 , Mojtaba Jafarinia5 |
1- MSc, Department of Genetics, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran
2- Professor, Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mhashemi@iautmu.ac.ir
3- Professor, Department of Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences University, Tehran, Iran
4- Associate professor, Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
5- Assistant professor, Department of Genetics, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran |
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Abstract: (2474 Views) |
Background: Most drugs used by pregnant women can pass through the placenta and expose embryos and developing embryos to teratogenic effects. Due to the increased cellular damage of Bax gene expression, the effect of cholorodiazepoxide use during pregnancy on Bax gene expression in the hippocampus of neonatal rats was investigated.
Materials and methods: After confirmation of pregnancy in Wistar female rats, intraperitoneal injection of chlordiazepoxide was administered at a dose of 10 mg/ kg daily for 21 days in the experimental group. Two weeks after birth, the infant's brain was removed from the skull. After isolating the CA1 region of the neonatal hippocampus, the expression of the Bax proapoptotic gene was examined and compared with the control and carrier group (saline).
Results: The level of gene expression was analyzed and propoptotic Bax gene showed significant increase in experimental group compared to control group.
Conclusion: The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats. |
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| Keywords: Gene expression, Chlorodiazepoxide, Rat, Hippocampus |
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Full-Text [PDF 350 kb]
(3541 Downloads)
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Semi-pilot: Experimental |
Subject:
Genetic
Received: 2019/02/16 | Accepted: 2019/04/7 | Published: 2020/06/28
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| References |
1. Yang Y, Raine A. Prefrontal structural and functional brain imaging findings in antisocial, violent, and psychopathic individuals: a meta-analysis. Psychiatry Research: Neuroimaging 2009;174:81-8. [ DOI:10.1016/j.pscychresns.2009.03.012] 2. Alvarez JA, Emory E. Executive function and the frontal lobes: a meta-analytic review. Neuropsychol Rev 2006;16:17-42. [ DOI:10.1007/s11065-006-9002-x] 3. Mander BA, Rao V, Lu B, Saletin JM, Lindquist JR, Ancoli-Israel S. Prefrontal atrophy, disrupted NREM slow waves and impaired hippocampal-dependent memory in aging. Nat Neurosci 2013;16:357-64. [ DOI:10.1038/nn.3324] 4. Dorph-Petersen K, Pierri J, Perel J, Sun Z, Sampson A, Lewis D. The influence of chronic exposure to antipsychotic medications on brain size before and after tissue fixation: a comparison of haloperidol and olanzapine in macaque monkeys. Neuropsychopharmacology 2005;30:1649-61. [ DOI:10.1038/sj.npp.1300710] 5. Liston C, Miller MM, Goldwater DS, Radley JJ, Rocher AB, Hof PR. Stress-induced alterations in prefrontal cortical dendritic morphology predict selective impairments in perceptual attentional set-shifting. J Neurosci 2006;26:7870-4. [ DOI:10.1523/JNEUROSCI.1184-06.2006] 6. Cecil KM, Brubaker CJ, Adler CM, Dietrich KN, Altaye M, Egelhoff JC, et al. Decreased brain volume in adults with childhood lead exposure. PLoS Med. 2008; 5: e112. [ DOI:10.1371/journal.pmed.0050112] 7. Iqbal M, Aneja A, Fremont W. Effects of chlordiazepoxide (Librium) during pregnancy and lactation. Conn Med 2003;67:259-62. 8. Iqbal M, Sobhan T, Ryals T. Effects of commonly used benzodiazepines on the fetus, the neonate, and the nursing infant. Psychiatr Serv 2002;53:39-49. [ DOI:10.1176/appi.ps.53.1.39] 9. Hassanzadeh P, Hassanzadeh A. Effects of psychotropic drugs on nerve growth factor protein levels in the rat brain. Physiol Pharmacol 2009;13:244-52. 10. Chaouloff F, Durand M, Mormède P. Anxiety- and activity-related effects of diazepam and chlordiazepoxide in the rat light/dark and dark/light tests. Behav Brain Res 1997;85:27-35. [ DOI:10.1016/S0166-4328(96)00160-X] 11. Schechter MD, Lovano DM. Ethanol-chlordiazepoxide interactions in the rat. Pharmacol Biochem Behav 1985; 23:927-30 [ DOI:10.1016/0091-3057(85)90094-2] 12. Kellogg CK. Benzodiazepines: influence on the developing brain. Prog Brain Res 1988;73:207-28. [ DOI:10.1016/S0079-6123(08)60506-3] 13. Katzung BG, Masters SB, Trevor AJ, Editors. Basic & clinical pharmacology. New York, NY, USA: Lange Medical Books/McGraw-Hill; 2012. 14. Zhang T, Cao EH, Li JF- A laser scanning confocal microscopy method. Simultaneous detection of intracellular ca2+ and apoptosis using Fluo- 3 and Hoechst 33342. Anal Quant Cytol Histol 2000; 22: 93-7. 15. Zhang F, Li C, Wang R, Han D, Zhang Q-G, Zhou C, et al. Activation of GABA receptors attenuates neuronal apoptosis through inhibiting the tyrosine phosphorylation of NR2A by Src after cerebral ischemia and reperfusion. Neuroscience 2007;150:938-49 [ DOI:10.1016/j.neuroscience.2007.09.070] 16. Glykys J, Staley KJ. Diazepam effect during early neonatal development correlates with neuronal Cl−. Ann Clin Transl Neurol 2015;2:1055-1070. [ DOI:10.1002/acn3.259] 17. Hartz SC, Heinonen OP, Shapiro S, Siskind V, Slone D. Antenatal exposure to meprobamate and chlordiazepoxide in relation to malformations, mental development, and childhood mortality. N Engl J Med 1975;292:726-8. [ DOI:10.1056/NEJM197504032921405] 18. Hashemi I, Entezari M, Zarindast MR. Investigating the gene expression level of Bad and Bcl-xl following cholestasis and curcumin treatment in the striatum area of male rat. Medical Sciences Journal of Islamic Azad University Tehran Medical Branch 2017;27:244-251. [In Persian]
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Dinarvand A, Hashemi M, Dinarvand R, Movaseghi S, Jafarinia M. The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus. MEDICAL SCIENCES 2020; 30 (2) :134-140 URL: http://tmuj.iautmu.ac.ir/article-1-1771-en.html
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