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:: Volume 33, Issue 4 (winter 2023) ::
MEDICAL SCIENCES 2023, 33(4): 338-346 Back to browse issues page
Investigating the effect of intramyocardial injection of DNAzyme-containing nisosome of NF-κB gene on the level of cardiac tissue NF-κB protein expression in cardiac ischemia-reperfusion of male rats
Seyede Bahar Hosseinian1 , Nahid Aboutaleb2 , Maryam Naseroleslami3 , Neda Mousavi Niri 4
1- Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4- Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mousaviniri@gmail.com
Abstract:   (357 Views)
Background: NF-κB factor is activated by ischemic damage and reperfusion and has a crucial role in transcription of several genes. Applying DNAzyme is one of the novel therapeutic means. Niosome is a new drug delivery system that is controllable ae used targeted. Applying DNAzyme-containing nanoniosome to diminish the expression of NF-κB protein after cardiac ischemia.
Materials and methods: In the current study, twenty-five male rats were assigned into 5 groups: 1- Control group, 2- Ischemic induced group, 3- Post ischemic DNAzyme receiving group, 4- Post ischemic DNAzyme-bearing nanoniosome receiving group, and 5- Post ischemic nanoniosome receiving group. After 72 hours, western blotting was performed on rats’ hearts to assess expression of NF-κB protein in treated groups.
Results: post ischemic treatment with DNAzyme-bearing nanoniosome led to considerably decrease of NF-κB expression compared to ischemic rats (P<0.05).
Conclusion: Results demonstrated the efficiency of DNAzyme-containing nanoniosome on NF-κB protein after cardiac ischemia. Therefore it is propounded as a curative method for cardiac ischemia
Keywords: DNAzyme, NF-κB, cardiac ischemia
Full-Text [PDF 517 kb]   (252 Downloads)    
Semi-pilot: Experimental | Subject: Molecular Biology
Received: 2023/05/10 | Accepted: 2023/06/28 | Published: 2023/12/1
References
1. Amoabediny G, Haghiralsadat F, Naderinezhad S, Helder MN, Akhoundi Kharanaghi E, Mohammadnejad Arough J, et al. Overview of preparation methods of polymeric and lipid-based (niosome, solid lipid, liposome) nanoparticles: A comprehensive review. Int J Polym Mater 2018;67:383-400. [DOI:10.1080/00914037.2017.1332623]
2. Somasuntharam I, Yehl K, Carroll SL, Maxwell JT, Martinez MD, Che P-L, et al. Knockdown of TNF-α by DNAzyme gold nanoparticles as an anti-inflammatory therapy for myocardial infarction. Biomaterials 2016;83:12-22. [DOI:10.1016/j.biomaterials.2015.12.022]
3. Benson VL, Khachigian LM, Lowe HC. DNAzymes and cardiovascular disease. Br J Pharmacol 2008;154:741-8. [DOI:10.1038/bjp.2008.145]
4. Schreiber T, Salhöfer L, Quinting T, Fandrey J. Things get broken: the hypoxia-inducible factor prolyl hydroxylases in ischemic heart disease. Basic Res Cardiol 2019;114:16. [DOI:10.1007/s00395-019-0725-2]
5. Frantz S, Tillmanns J, Kuhlencordt PJ, Schmidt I, Adamek A, Dienesch C, et al, Bauersachs J. Tissue-specific effects of the nuclear factor kappaB subunit p50 on myocardial ischemia-reperfusion injury. Am J Pathol 2007;171:507-12. [DOI:10.2353/ajpath.2007.061042]
6. Skourtis D, Stavroulaki D, Athanasiou V, Fragouli PG, Iatrou H. Nanostructured Polymeric, Liposomal and Other Materials to Control the Drug Delivery for Cardiovascular Diseases. Pharmaceutics 2020;12:1160. [DOI:10.3390/pharmaceutics12121160]
7. Lu D, Thum T. RNA-based diagnostic and therapeutic strategies for cardiovascular disease. Nat Rev Cardiol 2019;16:661-674. [DOI:10.1038/s41569-019-0218-x]
8. Sala V, Bergerone S, Gatti S, Gallo S, Ponzetto A, Ponzetto C, Crepaldi T. MicroRNAs in myocardial ischemia: identifying new targets and tools for treating heart disease. New frontiers for miR-medicine. Cell Mol Life Sci 2014;7:1439-52. [DOI:10.1007/s00018-013-1504-0]
9. Parlakpinar H, Orum M, Sagir M. Pathophysiology of myocardial ischemia reperfusion injury: a review. Med Sci 2013;2:935-54. [DOI:10.5455/medscience.2013.02.8082]
10. Zimmermann AC, White IM, Kahn JD. Nucleic acid-cleaving catalytic DNA for sensing and therapeutics. Talanta 2020;211:120709. [DOI:10.1016/j.talanta.2019.120709]
11. Ag Seleci D, Seleci M, Walter J-G, Stahl F, Scheper T. Niosomes as nanoparticular drug carriers: fundamentals and recent applications. J Nanomater 2016;2016. [DOI:10.1155/2016/7372306]
12. Latanich CA, Toledo-Pereyra LH. Searching for NF-kappaB-based treatments of ischemia reperfusion injury. J Invest Surg 2009;22301-15. [DOI:10.1080/08941930903040155]
13. Wang X, Zhang L, Ding N, Yang X, Zhang J, He J, et al. Identification and characterization of DNAzymes targeting DNA methyltransferase I for suppressing bladder cancer proliferation. Biochem Biophys Res Commun 2015;461:329-33. [DOI:10.1016/j.bbrc.2015.04.033]
14. 14 Zhao L, Yang XR, Han X. MicroRNA-146b induces the PI3K/Akt/NF-κB signaling pathway to reduce vascular inflammation and apoptosis in myocardial infarction by targeting PTEN. Exp Ther Med 2019;17:1171-1181. [DOI:10.3892/etm.2018.7087]
15. Lake RJ, Yang Z, Zhang J, Lu Y. DNAzymes as Activity-Based Sensors for Metal Ions: Recent Applications, Demonstrated Advantages, Current Challenges, and Future Directions. Acc Chem Res 2019;52:3275-3286. [DOI:10.1021/acs.accounts.9b00419]
16. Pardakhty A, Moazeni E. Nano-niosomes in drug, vaccine and gene delivery: a rapid overview. Nanomed J 2013;1:1-12.
17. Farrokhi F, Karami Z, Esmaeili-Mahani S, Heydari A. Delivery of DNAzyme targeting c-Myc gene using β-cyclodextrin polymer nanocarrier for therapeutic application in human breast cancer cell line. J Drug Deliv Sci Technol 2018;47:477-84. [DOI:10.1016/j.jddst.2018.08.015]
18. Xiang G, Schuster MD, Seki T, Witkowski P, Eshghi S, Itescu S. Downregulated expression of plasminogen activator inhibitor-1 augments myocardial neovascularization and reduces cardiomyocyte apoptosis after acute myocardial infarction. J Am Coll Cardiol 2005;46:536-41. [DOI:10.1016/j.jacc.2005.04.047]
19. Jiang T, You H, You D, Zhang L, Ding M, Yang B. A miR-1275 mimic protects myocardiocyte apoptosis by regulating the Wnt/NF-κB pathway in a rat model of myocardial ischemia-reperfusion-induced myocardial injury. Mol Cell Biochem 2020 ;466:129-137. [DOI:10.1007/s11010-020-03695-w]
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Hosseinian S B, Aboutaleb N, Naseroleslami M, Mousavi Niri N. Investigating the effect of intramyocardial injection of DNAzyme-containing nisosome of NF-κB gene on the level of cardiac tissue NF-κB protein expression in cardiac ischemia-reperfusion of male rats. MEDICAL SCIENCES 2023; 33 (4) :338-346
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Volume 33, Issue 4 (winter 2023) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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