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Investigating the Correlation between Dopa Decarboxylase (DDC) and Dopamine Beta-Hydroxylase (DBH) genes Expression in Parkinson's and schizophrenia patients
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Fatemeh Dahehpour1    , Abdolreza Sabokrouh 2, Mina Samsam Shariat3 |
1- MSc Student of Biochemistry, Department of Biochemistry and Biophysics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Biochemistry, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , sabokrouh22@gmail.com
3- Department of Biochemistry and Biophysics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran |
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Abstract: (300 Views) |
Background: Neuro degenerative diseases, such as schizophrenia and Parkinson, are the main disorders in the human brain. The changes in the expression of dopa decarboxylase (DDC) and dopamine beta hydroxylase (DBH) genes are main causes, which play an important role in the level of dopamine in the brain. In this study, the correlation between the expression levels of DDC and DBH and these diseases has been investigated.
Materials and methods: In this research, the target people were divided into three groups including group A (healthy people), group B (people with schizophrenia) and group C (people with Parkinson). Each group includes 15 individuals. After RNA extraction and cDNA synthesis, the expression level of DDC and DBH genes expression was determined through Real-time PCR.
Results: The DDC expression showed decreased value in group C 0.08(0.03, 0.25) compared to group A 12.81(3.92, 31.34), and the DBH had increased value in group C 3.18(1.39, 8.14) compared to group A 0.22(0.11, 0.42). On the contrary, the DDC expression in group B revealed increased value 7.06(3.34, 9.75) compared to group A 0.14(0.1,0.3) and the DBH expression in group B had decreased value 0.04(0.03,0.58) compared to group A 31.34(16.51,82.27).
Conclusion: Our results showed that DDC and DBH genes expression plays a role in the occurrence of the diseases. There was no significant relationship between the genes, so by monitoring the expression level of these two genes and finding drugs to regulate the expression of them, we can find a way to prevent and treat the diseases.
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| Keywords: Parkinson's disease, Schizophrenia disease, DDC gene, DBH gene. |
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Full-Text [PDF 343 kb]
(229 Downloads)
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Semi-pilot: case-control |
Subject:
Cilinical Biochemistry
Received: 2024/02/26 | Accepted: 2024/04/16 | Published: 2024/10/1
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1. Abbas Nejad Sh, Rezvani Z. Investigating the role of responsible genes in the monogenic form of Parkinson's disease and bioinformatics analysis of common mutations. J Facul Med Mashhad Uni Med Sci 2021;64: 3967-3997. [In Persian] 2. Nussbaum RL, Ellis CE. Alzheimer's disease and Parkinson's disease. N Engl J Med 2003; 348:1356-64. [ DOI:10.1056/NEJM2003ra020003] 3. Kalia LV, Lang AE. Parkinson's disease. Lancet 2015; 386:896-912. [ DOI:10.1016/S0140-6736(14)61393-3] 4. Schapira AHV, Chaudhuri KR, Jenner P. Non-motor features of Parkinson disease. Nat Rev Neurosci 2017;18:509. [ DOI:10.1038/nrn.2017.91] 5. Nutt JG. Pharmacokinetics and pharmacodynamics of levodopa. Mov Disord 2008;23:S580-4. [ DOI:10.1002/mds.22037] 6. Wilkins RC, Lis JT. GAGA factor binding to DNA via a single trinucleotide sequence element. Nucleic Acids Res 1998:2672-8. [ DOI:10.1093/nar/26.11.2672] 7. Borglum AD, Kirov G, Craddock N, Mors O, Muir W, Murray V, et al. Possible parent-of-origin effect of Dopa decarboxylase in susceptibility to bipolar affective disorder. Am J Med Genet B Neuropsychiatr Genet 2003:18-22. [ DOI:10.1002/ajmg.b.10030] 8. Alexoudi A, Shalash A, Knudsen K, Witt K, Mehdorn M, Volkmann J, et al. The medical treatment of patients with Parkinson's disease receiving subthalamic neurostimulation. Parkinsonism Relat Disord 2015; 21:555-60. [ DOI:10.1016/j.parkreldis.2015.03.003] 9. Ehgoetz Martens KA, Shine JM, Walton CC, Georgiades MJ, Gilat M, Hall JM, et al. Evidence for subtypes of freezing of gait in Parkinson's disease. Mov Disord 2018; 33:1174-1178. [ DOI:10.1002/mds.27417] 10. Patel KR, Cherian J, Gohil K, Atkinson D. Schizophrenia: overview and treatment options. P T 2014; 39:638-45. 11. Hany M, Rehman B, Azhar Y, Chapman J. Schizophrenia. In: StatPearls [Internet]. Treasure Island, FL: StatPearls Publishing; 2023. 12. Sun Z, Ma Y, Li W, He J, Li J, Yang X, et al. Associations between the DBH gene, plasma dopamine β-hydroxylase activity and cognitive measures in Han Chinese patients with schizophrenia. Schizophr Res 2018;193:58-63. [ DOI:10.1016/j.schres.2017.06.028] 13. Ghosh A, Sadhukhan T, Giri S, Biswas A, Das SK, Ray K, Ray J. Dopamine β Hydroxylase (DBH) is a potential modifier gene associated with Parkinson's disease in Eastern India. Neurosci Lett 2019; 706:75-80. [ DOI:10.1016/j.neulet.2019.05.015] 14. Devos D, Lejeune S, Cormier-Dequaire F, Tahiri K, Charbonnier-Beaupel F, Rouaix N, et al. Dopa-decarboxylase gene polymorphisms affect the motor response to L-dopa in Parkinson's disease. Parkinsonism Relat Disord 2014; 20:170-5. [ DOI:10.1016/j.parkreldis.2013.10.017] 15. Babić Leko M, Nikolac Perković M, Klepac N, Švob Štrac D, Borovečki F, Pivac N, e al. Relationships of Cerebrospinal Fluid Alzheimer's Disease Biomarkers and COMT, DBH, and MAOB Single Nucleotide Polymorphisms. J Alzheimers Dis 2020; 73:135-145. [ DOI:10.3233/JAD-190991] 16. Graziano C, Wischmeijer A, Pippucci T, Fusco C, Diquigiovanni C, Nõukas M, et al. Syndromic intellectual disability: a new phenotype caused by an aromatic amino acid decarboxylase gene (DDC) variant. Gene 2015;559:144-8. [ DOI:10.1016/j.gene.2015.01.026] 17. Kopecková M, Paclt I, Goetz P. Polymorphisms and low plasma activity of dopamine-beta-hydroxylase in ADHD children. Neuro Endocrinol Lett 2006;27:748-54. 18. Artemaki PI, Papatsirou M, Boti MA, Adamopoulos PG, Christodoulou S, Vassilacopoulou D, et al. Revised Exon Structure of l-DOPA Decarboxylase (DDC) Reveals Novel Splice Variants Associated with Colorectal Cancer Progression. Int J Mol Sci 2020;21:8568. [ DOI:10.3390/ijms21228568] 19. Toma C, Hervás A, Balmaña N, Salgado M, Maristany M, Vilella E, et al. Neurotransmitter systems and neurotrophic factors in autism: association study of 37 genes suggests involvement of DDC. World J Biol Psychiatry 2013; 14:516-27. [ DOI:10.3109/15622975.2011.602719] 20. Koutalellis G, Stravodimos K, Avgeris M, Mavridis K, Scorilas A, Lazaris A, et al. L-dopa decarboxylase (DDC) gene expression is related to outcome in patients with prostate cancer. BJU Int 2012; 110:E267-73. [ DOI:10.1111/j.1464-410X.2012.11152.x] 21. Tremmel E, Kuhn C, Kaltofen T, Vilsmaier T, Mayr D, Mahner S, et al. L-Dopa-Decarboxylase (DDC) Is a Positive Prognosticator for Breast Cancer Patients and Epinephrine Regulates Breast Cancer Cell (MCF7 and T47D) Growth In Vitro According to Their Different Expression of Gi- Protein- Coupled Receptors. Int J Mol Sci 2020; 21:9565. [ DOI:10.3390/ijms21249565] |
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Dahehpour F, Sabokrouh A, Samsam Shariat M. Investigating the Correlation between Dopa Decarboxylase (DDC) and Dopamine Beta-Hydroxylase (DBH) genes Expression in Parkinson's and schizophrenia patients. MEDICAL SCIENCES 2024; 34 (3) :255-263 URL: http://tmuj.iautmu.ac.ir/article-1-2228-en.html
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