Background: Pseudomonas aeruginosa is a common hospital-acquired bacterium which has ability to produce fluorescent pigment like pyorubin and nonfluorescent pigment like pyocyanine. The aim of this study was exciting of production of pyoverdin pigments as biomarker for quick detection of this bacterium in foods and hygienic products.
Materials and Methods: In this research, different concentrations of cadmium sulfate were used for showing the exciting of pyoverdin pigments. Pigment type was evaluated according to the maximum absorbance and its solubility in water, acetic acid and chloroform. Also, bacterium growth pattern was determined according to pigment production in optimal conditions of agitation, temperature, cultivate media, sources of carbon and nitrogen. Relation between pigment production and number of bacteria was calculated by absorbance and cell accumulation. Bacteria tracking was investigated using pigment production as biomarker in foods (spaghetti and apple juice) and hygienic products (hand and dish washing liquids) and compared to standard method.
Results: This study showed maximum absorbance of extracted pigment at 403 nm and its solubility in water and acetic acid. Production of fluorescent pigment in bacterium was observed at concentrations 0.3-0.5 mM of cadmium sulfate. Maximum production of florescent pigments based on cell accumulation was established by agitation in 150 rpm, temperature of 35oC, 0.5 mM solution of cadmium and concentration of 330 mgml-1of 1% sucrose and potassium nitrate.
Conclusion: Monitoring of Pseudomonas aeruginosa in contaminated foods and hygienic products based on fluorescent pigments production was reduced from 72 to 20 hours.